An epidemic CC1-MRSA-IV clone yields false-negative test results in molecular MRSA identification assays: a note of caution, Austria, Germany, Ireland, 2020.
Monecke, Stefan; König, Elisabeth; Earls, Megan R; Leitner, Eva; Müller, Elke; Wagner, Gabriel E; Poitz, David M; Jatzwauk, Lutz; Vremerǎ, Teodora; Dorneanu, Olivia S; Simbeck, Alexandra; Ambrosch, Andreas; Zollner-Schwetz, Ines; Krause, Robert; Ruppitsch, Werner; Schneider-Brachert, Wulf; Coleman, David C; Steinmetz, Ivo; Ehricht, Ralf
We investigated why a clinical meticillin-resistant Staphylococcus aureus (MRSA) isolate yielded false-negative results with some commercial PCR tests for MRSA detection. We found that an epidemic European CC1-MRSA-IV clone generally exhibits this behaviour. The failure of the assays was attributable to a large insertion in the orfX/SCCmec integration site. To ensure the reliability of molecular MRSA tests, it is vital to monitor emergence of new SCCmec types and junction sites.
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